This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029895
PrimePCR™ PreAmp for SYBR® Green Assay: LUM, Human
PrimePCR™ Template for SYBR® Green Assay: LUM, Human
This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin biglycan fibromodulin keratocan epiphycan and osteoglycin. In these bifunctional molecules the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth corneal transparency and epithelial cell migration and tissue repair. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.