PrimePCR™ SYBR® Green Assay: DNAH9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029850

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DNAH9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DNAH9, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015314
Assay Design:   Intron-spanning
Chromosome Location:   17:11795163-11797739question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000262442 ENST00000413703 ENST00000396001 ENST00000454412 ENST00000361801

Gene Information

This gene encodes the heavy chain subunit of axonemal dynein a large multi-subunit molecular motor. Axonemal dynein attaches to microtubules and hydrolyzes ATP to mediate the movement of cilia and flagella. The gene expresses at least two transcript variants; additional variants have been described but their full length nature has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   DNAH9
Gene Name:   dynein, axonemal, heavy chain 9
Aliases:   DNAH17L, DNEL1, DYH9, Dnahc9, HL-20, HL20, KIAA0357
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000007174
Entrez:   1770
Chromosome Mapping:   17p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.790000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download