PrimePCR™ SYBR® Green Assay: ACO1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029848

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015310
Assay Design:   Intron-spanning
Chromosome Location:   9:32420990-32424549question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000309951 ENST00000379923 ENST00000379921 ENST00000432017 ENST00000541043

Gene Information

Aconitase 1 also known as iron regulatory element binding protein 1 (IREB1) is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity and hence grouped with the aconitase family of enzymes. [provided by RefSeq Jul 2008]

Gene Symbol:   ACO1
Gene Name:   aconitase 1, soluble
Aliases:   ACONS, IREB1, IREBP, IREBP1, IRP1
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000122729
Entrez:   48
UniGene:   Hs.567229
Chromosome Mapping:   9p22-q32,9p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 35.030000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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