This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029834
PrimePCR™ PreAmp for SYBR® Green Assay: MEP1B, Human
PrimePCR™ Template for SYBR® Green Assay: MEP1B, Human
Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted whereas oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability renal gene expression profiles and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.