PrimePCR™ SYBR® Green Assay: KDM1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029832

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: KDM1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KDM1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015285
Assay Design:   Intron-spanning
Chromosome Location:   6:18218055-18222171question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000297792 ENST00000388869 ENST00000449850 ENST00000388870 ENST00000397244 ENST00000546309

Gene Information

Flavin-dependent histone demethylases such as KDM1B regulate histone lysine methylation an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al. 2009 [PubMed 19407342]).[supplied by OMIM Oct 2009]

Gene Symbol:   KDM1B
Gene Name:   lysine (K)-specific demethylase 1B
Aliases:   AOF1, C6orf193, DKFZp686I0412, FLJ33898, FLJ34109, FLJ43328, LSD2, bA204B7.3, dJ298J15.2
RefSeq:   NG_012137.1 NC_000006.11 NT_007592.15
Ensembl:   ENSG00000165097
Entrez:   221656
Chromosome Mapping:   6p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.930000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download