PrimePCR™ SYBR® Green Assay: SYTL5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029792

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SYTL5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SYTL5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015227
Assay Design:   Intron-spanning
Chromosome Location:   X:37984712-37985893question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000297875 ENST00000357972 ENST00000456733

Gene Information

The protein encoded by this gene belongs to the synaptotagmin-like (Slp) protein family which contains a unique homology domain at the N-terminus referred to as the Slp homology domain (SHD). The SHD functions as a binding site for Rab27A which plays a role in protein transport. Expression of this gene is restricted to placenta and liver suggesting that it might be involved in Rab27A-dependent membrane trafficking in specific tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2009]

Gene Symbol:   SYTL5
Gene Name:   synaptotagmin-like 5
Aliases:   slp5
RefSeq:   NC_000023.10 NT_079573.4 NG_013265.1
Ensembl:   ENSG00000147041
Entrez:   94122
Chromosome Mapping:   Xp21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.480000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download