This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: CDIPT, Human
PrimePCR™ Template for SYBR® Green Assay: CDIPT, Human
Phosphatidylinositol breakdown products are ubiquitous second messengers that function downstream of many G protein-coupled receptors and tyrosine kinases regulating cell growth calcium metabolism and protein kinase C activity. Two enzymes CDP-diacylglycerol synthase and phosphatidylinositol synthase are involved in the biosynthesis of phosphatidylinositol. Phosphatidylinositol synthase a member of the CDP-alcohol phosphatidyl transferase class-I family is an integral membrane protein found on the cytoplasmic side of the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.