PrimePCR™ SYBR® Green Assay: WDR16, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029736

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015144
Assay Design:   Intron-spanning
Chromosome Location:   17:9545108-9546418question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000352665 ENST00000396219 ENST00000299764

Gene Information

WD repeat-containing proteins such as WDR16 play crucial roles in a wide range of physiologic functions including signal transduction RNA processing remodeling the cytoskeleton regulation of vesicular traffic and cell division (Silva et al. 2005 [PubMed 15967112]).[supplied by OMIM Mar 2008]

Gene Symbol:   WDR16
Gene Name:   WD repeat domain 16
Aliases:   FLJ37528, WDRPUH
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000166596
Entrez:   146845
UniGene:   Hs.232270
Chromosome Mapping:   17p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.600000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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