This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029699
PrimePCR™ PreAmp for SYBR® Green Assay: PPP1R14C, Human
PrimePCR™ Template for SYBR® Green Assay: PPP1R14C, Human
The degree of protein phosphorylation is regulated by a balance of protein kinase and phosphatase activities. Protein phosphatase-1 (PP1; see MIM 176875) is a signal-transducing phosphatase that influences neuronal activity protein synthesis metabolism muscle contraction and cell division. PPP1R14C is an inhibitor of PP1 (Liu et al. 2002 [PubMed 11812771]).[supplied by OMIM Feb 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.