PrimePCR™ SYBR® Green Assay: PTGR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029682

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PTGR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PTGR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015062
Assay Design:   Intron-spanning
Chromosome Location:   9:114355206-114359710question
Amplicon Length:   187
Splice Variants Targeted:   ENST00000309195 ENST00000374324 ENST00000374313 ENST00000374308 ENST00000538962 ENST00000407693 ENST00000238248

Gene Information

This gene encodes an enzyme that is involved in the inactivation of the chemotactic factor leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq Mar 2009]

Gene Symbol:   PTGR1
Gene Name:   prostaglandin reductase 1
Aliases:   FLJ99229, LTB4DH, MGC34943, PGR1, ZADH3
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000106853
Entrez:   22949
Chromosome Mapping:   9q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.580000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download