This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029648
PrimePCR™ PreAmp for SYBR® Green Assay: JARID2, Human
PrimePCR™ Template for SYBR® Green Assay: JARID2, Human
This gene is an ortholog of the mouse jumonji gene which encodes a nuclear protein essential for mouse embryogenesis including neural tube formation. Overexpression of mouse jumonji negatively regulates cell proliferation. The jumonji proteins contain a DNA-binding domain called an AT-rich interaction domain (ARID) and share regions of similarity with human retinoblastoma-binding protein-2 and the human SMCX protein. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.