PrimePCR™ SYBR® Green Assay: JARID2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029648

List Price:    $130.00
Your Price:   Log In
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014998
Assay Design:   Intron-spanning
Chromosome Location:   6:15504751-15507461question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000341776 ENST00000541660 ENST00000397311

Gene Information

This gene is an ortholog of the mouse jumonji gene which encodes a nuclear protein essential for mouse embryogenesis including neural tube formation. Overexpression of mouse jumonji negatively regulates cell proliferation. The jumonji proteins contain a DNA-binding domain called an AT-rich interaction domain (ARID) and share regions of similarity with human retinoblastoma-binding protein-2 and the human SMCX protein. [provided by RefSeq Jul 2008]

Gene Symbol:   JARID2
Gene Name:   jumonji, AT rich interactive domain 2
Aliases:   JMJ
RefSeq:   NC_000006.11 NG_009309.1 NT_007592.15
Ensembl:   ENSG00000008083
Entrez:   3720
UniGene:   Hs.269059
Chromosome Mapping:   6p24-p23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.470000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.