This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029635
PrimePCR™ PreAmp for SYBR® Green Assay: ST8SIA4, Human
PrimePCR™ Template for SYBR® Green Assay: ST8SIA4, Human
The protein encoded by this gene catalyzes the polycondensation of alpha-28-linked sialic acid required for the synthesis of polysialic acid a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). The encoded protein which is a member of glycosyltransferase family 29 is a type II membrane protein that may be present in the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.