This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029616
PrimePCR™ PreAmp for SYBR® Green Assay: CLDN16, Human
PrimePCR™ Template for SYBR® Green Assay: CLDN16, Human
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene a member of the claudin family is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys specifically in the thick ascending limb of Henle where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28. [provided by RefSeq Jun 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.