This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029613
PrimePCR™ PreAmp for SYBR® Green Assay: COL17A1, Human
PrimePCR™ Template for SYBR® Green Assay: COL17A1, Human
This gene encodes the alpha chain of type XVII collagen. Unlike most collagens collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form referred to as either ectodomain or LAD-1 is generated by proteolytic processing of the full length form. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.