PrimePCR™ SYBR® Green Assay: COL17A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029613

List Price:    $134.00
Your Price:   Log In
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014928
Assay Design:   Intron-spanning
Chromosome Location:   10:105797394-105798199question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000353479 ENST00000369733 ENST00000541872

Gene Information

This gene encodes the alpha chain of type XVII collagen. Unlike most collagens collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form referred to as either ectodomain or LAD-1 is generated by proteolytic processing of the full length form. [provided by RefSeq Jul 2008]

Gene Symbol:   COL17A1
Gene Name:   collagen, type XVII, alpha 1
Aliases:   BA16H23.2, BP180, BPAG2, FLJ60881, KIAA0204, LAD-1
RefSeq:   NC_000010.10 NG_007069.1 NT_030059.13
Ensembl:   ENSG00000065618
Entrez:   1308
UniGene:   Hs.117938
Chromosome Mapping:   10q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.730000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download