PrimePCR™ SYBR® Green Assay: FNBP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FNBP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FNBP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014809
Assay Design:   Intron-spanning
Chromosome Location:   9:132740777-132741637question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000372416 ENST00000449089 ENST00000355681 ENST00000446176 ENST00000420781 ENST00000372415

Gene Information

The protein encoded by this gene is a member of the formin-binding-protein family. The protein contains an N-terminal Fer/Cdc42-interacting protein 4 (CIP4) homology (FCH) domain followed by a coiled-coil domain a proline-rich motif a second coiled-coil domain a Rho family protein-binding domain (RBD) and a C-terminal SH3 domain. This protein binds sorting nexin 2 (SNX2) tankyrase (TNKS) and dynamin; an interaction between this protein and formin has not been demonstrated yet in human. [provided by RefSeq Jul 2008]

Gene Symbol:   FNBP1
Gene Name:   formin binding protein 1
Aliases:   FBP17, KIAA0554, MGC126804
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000187239
Entrez:   23048
Chromosome Mapping:   9q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.740000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download