This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029556
PrimePCR™ PreAmp for SYBR® Green Assay: ZNF384, Human
PrimePCR™ Template for SYBR® Green Assay: ZNF384, Human
This gene encodes a C2H2-type zinc finger protein which may function as a transcription factor. This gene also contains long CAG trinucleotide repeats that encode consecutive glutamine residues. The protein appears to bind and regulate the promoters of the extracellular matrix genes MMP1 MMP3 MMP7 and COL1A1. Studies in mouse suggest that nuclear matrix transcription factors (NP/NMP4) may be part of a general mechanical pathway that couples cell construction and function during extracellular matrix remodeling. Alternative splicing results in multiple transcript variants. Recurrent rearrangements of this gene with the Ewing's sarcoma gene EWSR1 on chromosome 22 or with the TAF15 gene on chromosome 17 or with the TCF3 (E2A) gene on chromosome 19 have been observed in acute leukemia. A related pseudogene has been identified on chromosome 7. [provided by RefSeq Apr 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.