PrimePCR™ SYBR® Green Assay: GLDC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GLDC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GLDC, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014770
Assay Design:   Intron-spanning
Chromosome Location:   9:6602132-6604650question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000321612

Gene Information

Degradation of glycine is brought about by the glycine cleavage system which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase) H protein (a lipoic acid-containing protein) T protein (a tetrahydrofolate-requiring enzyme) and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).[provided by RefSeq Jan 2010]

Gene Symbol:   GLDC
Gene Name:   glycine dehydrogenase (decarboxylating)
Aliases:   GCE, GCSP, HYGN1, MGC138198, MGC138200
RefSeq:   NC_000009.11 NG_016397.1 NT_008413.18
Ensembl:   ENSG00000178445
Entrez:   2731
Chromosome Mapping:   9p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.650000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download