This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029540
PrimePCR™ PreAmp for SYBR® Green Assay: COL27A1, Human
PrimePCR™ Template for SYBR® Green Assay: COL27A1, Human
Fibrillar collagens such as COL27A1 compose one of the most ancient families of extracellular matrix molecules. They form major structural elements in extracellular matrices of cartilage skin and tendon (Boot-Handford et al. 2003 [PubMed 12766169]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.