This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029539
PrimePCR™ PreAmp for SYBR® Green Assay: PLXNC1, Human
PrimePCR™ Template for SYBR® Green Assay: PLXNC1, Human
This gene encodes a member of the plexin family. Plexins are transmembrane receptors for semaphorins a large family of proteins that regulate axon guidance cell motility and migration and the immune response. The encoded protein and its ligand regulate melanocyte adhesion and viral semaphorins may modulate the immune response by binding to this receptor. The encoded protein may be a tumor suppressor protein for melanoma. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq Jan 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.