PrimePCR™ SYBR® Green Assay: BAZ1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BAZ1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BAZ1A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014749
Assay Design:   Intron-spanning
Chromosome Location:   14:35242887-35243616question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000358716 ENST00000382422 ENST00000543083 ENST00000360310

Gene Information

The BAZ1A gene encodes the accessory subunit of the ATP-dependent chromatin assembly factor (ACF) a member of the ISWI ('imitation switch') family of chromatin remodeling complexes (summarized by Racki et al. 2009 [PubMed 20033039]).[supplied by OMIM Apr 2010]

Gene Symbol:   BAZ1A
Gene Name:   bromodomain adjacent to zinc finger domain, 1A
Aliases:   ACF1, DKFZp586E0518, FLJ14383, WALp1, WCRF180, hACF1
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000198604
Entrez:   11177
Chromosome Mapping:   14q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.600000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download