PrimePCR™ SYBR® Green Assay: STAB2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029537

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014748
Assay Design:   Intron-spanning
Chromosome Location:   12:104067808-104069795question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000388887 ENST00000258495

Gene Information

This gene encodes a large transmembrane receptor protein which may function in angiogenesis lymphocyte homing cell adhesion or receptor scavenging. The protein contains 7 fasciclin 15 epidermal growth factor (EGF)-like and 2 laminin-type EGF-like domains as well as a C-type lectin-like hyaluronan-binding Link module. The protein is primarily expressed on sinusoidal endothelial cells of liver spleen and lymph node. The receptor has been shown to bind and endocytose ligands such as hyaluronan low density lipoprotein Gram-positive and Gram-negative bacteria and advanced glycosylation end products. Supporting its possible role as a scavenger receptor the protein has been shown to cycle between the plasma membrane and lysosomes. [provided by RefSeq Jul 2008]

Gene Symbol:   STAB2
Gene Name:   stabilin 2
Aliases:   DKFZp434E0321, FEEL2, FELE-2, FELL2, FEX2, HARE
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000136011
Entrez:   55576
UniGene:   Hs.408249
Chromosome Mapping:   12q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.360000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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