PrimePCR™ SYBR® Green Assay: CPSF7, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014724
Assay Design:   Intron-spanning
Chromosome Location:   11:61187977-61188943question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000340437 ENST00000394888 ENST00000439958 ENST00000448745 ENST00000477890 ENST00000539952 ENST00000544585 ENST00000413232 ENST00000541963 ENST00000450000 ENST00000449811 ENST00000413184

Gene Information

Description Not Available

Gene Symbol:   CPSF7
Gene Name:   cleavage and polyadenylation specific factor 7, 59kDa
Aliases:   CFIm59, FLJ12529, FLJ39024, MGC9315
RefSeq:   NC_000011.9 NG_023393.1 NT_167190.1
Ensembl:   ENSG00000149532
Entrez:   79869
UniGene:   Hs.718984
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.170000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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