PrimePCR™ SYBR® Green Assay: SLC4A7, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014718
Assay Design:   Intron-spanning
Chromosome Location:   3:27427431-27431516question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000419036 ENST00000295736 ENST00000428386 ENST00000454389 ENST00000440156 ENST00000437179 ENST00000446700 ENST00000455077 ENST00000445684 ENST00000428179 ENST00000435667 ENST00000388777 ENST00000425128

Gene Information

Description Not Available

Gene Symbol:   SLC4A7
Gene Name:   solute carrier family 4, sodium bicarbonate cotransporter, member 7
Aliases:   DKFZp686H168, NBC2, NBC3, SBC2, SLC4A6
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000033867
Entrez:   9497
UniGene:   Hs.250072
Chromosome Mapping:   3p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.310000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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