This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029520
PrimePCR™ PreAmp for SYBR® Green Assay: SYN3, Human
PrimePCR™ Template for SYBR® Green Assay: SYN3, Human
This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains and they are implicated in synaptogenesis and the modulation of neurotransmitter release suggesting a potential role in several neuropsychiatric diseases. The protein encoded by this gene shares the synapsin family domain model with domains A C and E exhibiting the highest degree of conservation. The protein contains a unique domain J located between domains C and E. Based on this gene's localization to 22q12.3 a possible schizophrenia susceptibility locus and the established neurobiological roles of the synapsins this family member may represent a candidate gene for schizophrenia. The TIMP3 gene is located within an intron of this gene and is transcribed in the opposite direction. Alternative splicing of this gene results in multiple splice variants that encode different isoforms. [provided by RefSeq Oct 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.