PrimePCR™ SYBR® Green Assay: SYN3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029520

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014702
Assay Design:   Intron-spanning
Chromosome Location:   22:33327407-33376673question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000358763 ENST00000332840 ENST00000390686

Gene Information

This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains and they are implicated in synaptogenesis and the modulation of neurotransmitter release suggesting a potential role in several neuropsychiatric diseases. The protein encoded by this gene shares the synapsin family domain model with domains A C and E exhibiting the highest degree of conservation. The protein contains a unique domain J located between domains C and E. Based on this gene's localization to 22q12.3 a possible schizophrenia susceptibility locus and the established neurobiological roles of the synapsins this family member may represent a candidate gene for schizophrenia. The TIMP3 gene is located within an intron of this gene and is transcribed in the opposite direction. Alternative splicing of this gene results in multiple splice variants that encode different isoforms. [provided by RefSeq Oct 2008]

Gene Symbol:   SYN3
Gene Name:   synapsin III
Aliases:   Not Available
RefSeq:   NG_009117.1 NC_000022.10 NT_011520.12
Ensembl:   ENSG00000185666
Entrez:   8224
UniGene:   Hs.608750
Chromosome Mapping:   22q12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.620000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download