PrimePCR™ SYBR® Green Assay: SMG5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029499

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014661
Assay Design:   Intron-spanning
Chromosome Location:   1:156247774-156248804question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000361813 ENST00000368267

Gene Information

SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al. 2003 [PubMed 14636577]).[supplied by OMIM Mar 2008]

Gene Symbol:   SMG5
Gene Name:   Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans)
Aliases:   EST1B, FLJ12287, FLJ34864, KIAA1089, LPTS-RP1, LPTSRP1, SMG-5
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000198952
Entrez:   23381
UniGene:   Hs.516837
Chromosome Mapping:   1q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.900000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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