PrimePCR™ SYBR® Green Assay: LCOR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029489

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LCOR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LCOR, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014644
Assay Design:   Intron-spanning
Chromosome Location:   10:98709040-98714811question
Amplicon Length:   179
Splice Variants Targeted:   ENST00000371103 ENST00000371097 ENST00000356016 ENST00000540664

Gene Information

LCOR is a transcriptional corepressor widely expressed in fetal and adult tissues that is recruited to agonist-bound nuclear receptors through a single LxxLL motif also referred to as a nuclear receptor (NR) box (Fernandes et al. 2003 [PubMed 12535528]).[supplied by OMIM Mar 2008]

Gene Symbol:   LCOR
Gene Name:   ligand dependent nuclear receptor corepressor
Aliases:   FLJ38026, KIAA1795, MLR2, RP11-175O19.1
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000196233
Entrez:   84458
Chromosome Mapping:   10q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.620000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download