PrimePCR™ SYBR® Green Assay: ADCK3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029434

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ADCK3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ADCK3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014540
Assay Design:   Intron-spanning
Chromosome Location:   1:227172308-227174161question
Amplicon Length:   179
Splice Variants Targeted:   ENST00000366779 ENST00000366778 ENST00000366777 ENST00000366776 ENST00000366775 ENST00000405743 ENST00000458507 ENST00000433743

Gene Information

This gene encodes a mitochondrial protein similar to yeast ABC1 which functions in an electron-transferring membrane protein complex in the respiratory chain. It is not related to the family of ABC transporter proteins. Expression of this gene is induced by the tumor suppressor p53 and in response to DNA damage and inhibiting its expression partially suppresses p53-induced apoptosis. Alternatively spliced transcript variants have been found; however their full-length nature has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   ADCK3
Gene Name:   aarF domain containing kinase 3
Aliases:   ARCA2, CABC1, COQ8, MGC4849, SCAR9
RefSeq:   NC_000001.10 NG_012825.1 NT_167186.1
Ensembl:   ENSG00000163050
Entrez:   56997
Chromosome Mapping:   1q42.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 34.980000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download