This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029419
PrimePCR™ PreAmp for SYBR® Green Assay: CHEK2, Human
PrimePCR™ Template for SYBR® Green Assay: CHEK2, Human
In response to DNA damage and replication blocks cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated the encoded protein is known to inhibit CDC25C phosphatase preventing entry into mitosis and has been shown to stabilize the tumor suppressor protein p53 leading to cell cycle arrest in G1. In addition this protein interacts with and phosphorylates BRCA1 allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also mutations in this gene are thought to confer a predisposition to sarcomas breast cancer and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Apr 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.