PrimePCR™ SYBR® Green Assay: MYOC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029371

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MYOC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MYOC, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014420
Assay Design:   Intron-spanning
Chromosome Location:   1:171605750-171607841question
Amplicon Length:   175
Splice Variants Targeted:   ENST00000037502 ENST00000357746 ENST00000536591 ENST00000537133

Gene Information

MYOC encodes the protein myocilin which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues including the trabecular meshwork and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma. [provided by RefSeq Jul 2008]

Gene Symbol:   MYOC
Gene Name:   myocilin, trabecular meshwork inducible glucocorticoid response
Aliases:   GLC1A, GPOA, JOAG, JOAG1, TIGR, myocilin
RefSeq:   NC_000001.10 NG_008859.1 NT_004487.19
Ensembl:   ENSG00000034971
Entrez:   4653
Chromosome Mapping:   1q23-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.510000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download