PrimePCR™ SYBR® Green Assay: POGK, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014343
Assay Design:   Intron-spanning
Chromosome Location:   1:166810235-166815908question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000449930 ENST00000367876 ENST00000367875

Gene Information

The exact function of the protein encoded by this gene is not known. However this gene product contains a KRAB domain (which is involved in protein-protein interactions) at the N-terminus and a transposase domain at the C-terminus suggesting that it may belong to the family of DNA-mediated transposons in human. [provided by RefSeq Jul 2008]

Gene Symbol:   POGK
Gene Name:   pogo transposable element with KRAB domain
Aliases:   BASS2, KIAA1513, KIAA15131, KRBOX2
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143157
Entrez:   57645
UniGene:   Hs.432752
Chromosome Mapping:   1q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 35.330000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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