This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029319
PrimePCR™ PreAmp for SYBR® Green Assay: WFIKKN1, Human
PrimePCR™ Template for SYBR® Green Assay: WFIKKN1, Human
This gene encodes a secreted multidomain protein consisting of a signal peptide a WAP domain a follistatin domain an immunoglobulin domain two tandem Kunitz domains and an NTR domain. These domains have been implicated frequently in inhibition of various types of proteases suggesting that the encoded protein may be a multivalent protease inhibitor and may control the action of multiple types of serine proteases as well as metalloproteinases. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.