This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency
PrimePCR™ PreAmp for SYBR® Green Assay: GABRG3, Human
PrimePCR™ Template for SYBR® Green Assay: GABRG3, Human
Gamma-aminobutyric acid (GABA) receptors are a family of proteins involved in the GABAergic neurotransmission of the mammalian central nervous system. GABRG3 is a member of the GABA-A receptor gene family of heteromeric pentameric ligand-gated ion channels through which GABA the major inhibitory neurotransmitter in the mammalian brain acts. GABA-A receptors are the site of action of a number of important pharmacologic agents including barbiturates benzodiazepines and ethanol (summary by Whiting et al. 1999 [PubMed 10414349]). For additional general information about the GABA-A receptor gene family see GABRA1 (MIM 137160).[supplied by OMIM Dec 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.