This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029236
PrimePCR™ PreAmp for SYBR® Green Assay: GSTA5, Human
PrimePCR™ Template for SYBR® Green Assay: GSTA5, Human
The glutathione S-transferases (GST; EC 126.96.36.199) catalyze the conjugation of reduced glutathiones and a variety of electrophiles including many known carcinogens and mutagens. The cytosolic GSTs belong to a large superfamily with members located on different chromosomes. For additional information on GSTs see GSTA1 (MIM 138359).[supplied by OMIM Sep 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.