This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029206
PrimePCR™ PreAmp for SYBR® Green Assay: BAMBI, Human
PrimePCR™ Template for SYBR® Green Assay: BAMBI, Human
This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family whose members play important roles in signal transduction in many developmental and pathological processes. The encoded protein however is a pseudoreceptor lacking an intracellular serine/threonine kinase domain required for signaling. Similar proteins in frog mouse and zebrafish function as negative regulators of TGF-beta which has led to the suggestion that the encoded protein may function to limit the signaling range of the TGF-beta family during early embryogenesis. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.