PrimePCR™ SYBR® Green Assay: PDE6H, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PDE6H, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PDE6H, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014072
Assay Design:   Intron-spanning
Chromosome Location:   12:15131022-15134412question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000266395

Gene Information

This gene encodes the inhibitory (or gamma) subunit of the cone-specific cGMP phosphodiesterase which is a tetramer composed of two catalytic chains (alpha and beta) and two inhibitory chains (gamma). It is specifically expressed in the retina and is involved in the transmission and amplification of the visual signal. Mutations in this gene are associated with retinal cone dystrophy type 3A (RCD3A). [provided by RefSeq Mar 2010]

Gene Symbol:   PDE6H
Gene Name:   phosphodiesterase 6H, cGMP-specific, cone, gamma
Aliases:   RCD3
RefSeq:   NC_000012.11 NG_016859.1 NT_009714.17
Ensembl:   ENSG00000139053
Entrez:   5149
Chromosome Mapping:   12p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.540000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download