This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029169
PrimePCR™ PreAmp for SYBR® Green Assay: GHRH, Human
PrimePCR™ Template for SYBR® Green Assay: GHRH, Human
The protein encoded by this gene belongs to the glucagon family and is a preproprotein that is produced in the hypothalamus. The preproprotein is cleaved to form a 44 aa factor also called somatocrinin that acts to stimulate growth hormone release from the pituitary. Variant receptors for somatocrinin have been found in several types of tumors and antagonists of these receptors can inhibit the growth of the tumors. Defects in this gene are a cause of dwarfism while hypersecretion of the encoded protein is a cause of gigantism. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.