This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029168
PrimePCR™ PreAmp for SYBR® Green Assay: CORT, Human
PrimePCR™ Template for SYBR® Green Assay: CORT, Human
This gene encodes a neuropeptide that is structurally similar to somatostatin. It binds to all known somatostatin receptors and shares many pharmacological and functional properties with somatostatin including the depression of neuronal activity. However it also has many properties distinct from somatostatin such as induction of slow-wave sleep apparently by antagonism of the excitatory effects of acetylcholine on the cortex reduction of locomotor activity and activation of cation selective currents not responsive to somatostatin. The preproprotein undergoes further processing into multiple mature products. Read-through transcripts exist between this gene and the upstream APITD1 (apoptosis-inducing TAF9-like domain 1) gene as represented in GeneID:100526739. [provided by RefSeq Nov 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.