This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029164
PrimePCR™ PreAmp for SYBR® Green Assay: KCNE2, Human
PrimePCR™ Template for SYBR® Green Assay: KCNE2, Human
Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release heart rate insulin secretion neuronal excitability epithelial electrolyte transport smooth muscle contraction and cell volume. This gene encodes a member of the potassium channel voltage-gated isk-related subfamily. This member is a small integral membrane subunit that assembles with the KCNH2 gene product a pore-forming protein to alter its function. This gene is expressed in heart and muscle and the gene mutations are associated with cardiac arrhythmia. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.