This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029135
PrimePCR™ PreAmp for SYBR® Green Assay: CAMP, Human
PrimePCR™ Template for SYBR® Green Assay: CAMP, Human
This gene encodes a member of an antimicrobial peptide family characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide which is produced by extracellular proteolysis from the C-terminus. The encoded protein has several functions in addition to antimicrobial activity including cell chemotaxis immune mediator induction and inflammatory response regulation. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.