PrimePCR™ SYBR® Green Assay: CAMP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029135

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CAMP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CAMP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013982
Assay Design:   Intron-spanning
Chromosome Location:   3:48265095-48265912question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000296435

Gene Information

This gene encodes a member of an antimicrobial peptide family characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide which is produced by extracellular proteolysis from the C-terminus. The encoded protein has several functions in addition to antimicrobial activity including cell chemotaxis immune mediator induction and inflammatory response regulation. [provided by RefSeq Aug 2011]

Gene Symbol:   CAMP
Gene Name:   cathelicidin antimicrobial peptide
Aliases:   CAP-18, CAP18, CRAMP, FALL-39, FALL39, LL37
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000164047
Entrez:   820
Chromosome Mapping:   3p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.430000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download