PrimePCR™ SYBR® Green Assay: TFAP4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029132

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TFAP4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TFAP4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013978
Assay Design:   Intron-spanning
Chromosome Location:   16:4312586-4322759question
Amplicon Length:   188
Splice Variants Targeted:   ENST00000204517

Gene Information

Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain which is used for DNA binding and HLH and ZIP domains which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al. 1988 [PubMed 2833704]; Hu et al. 1990 [PubMed 2123466]).[supplied by OMIM Jul 2009]

Gene Symbol:   TFAP4
Gene Name:   transcription factor AP-4 (activating enhancer binding protein 4)
Aliases:   AP-4, bHLHc41
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000090447
Entrez:   7023
Chromosome Mapping:   16p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.540000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download