This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029132
PrimePCR™ PreAmp for SYBR® Green Assay: TFAP4, Human
PrimePCR™ Template for SYBR® Green Assay: TFAP4, Human
Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain which is used for DNA binding and HLH and ZIP domains which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al. 1988 [PubMed 2833704]; Hu et al. 1990 [PubMed 2123466]).[supplied by OMIM Jul 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.