This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: ARHGDIB, Human
PrimePCR™ Template for SYBR® Green Assay: ARHGDIB, Human
Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events including cell signaling proliferation cytoskeletal organization and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs) guanine nucleotide-releasing factors (GRFs) and GDP-dissociation inhibitors (GDIs). The GDIs including ARHGDIB decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al. 1993 [PubMed 8356058]).[supplied by OMIM Dec 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.