PrimePCR™ SYBR® Green Assay: HCRT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: HCRT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HCRT, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013873
Assay Design:   Intron-spanning
Chromosome Location:   17:40336411-40337417question
Amplicon Length:   161
Splice Variants Targeted:   ENST00000293330

Gene Information

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides orexin A and orexin B by proteolytic processing. Orexin A and orexin B which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2 function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior metabolism and homeostasis. [provided by RefSeq Jan 2010]

Gene Symbol:   HCRT
Gene Name:   hypocretin (orexin) neuropeptide precursor
Aliases:   NRCLP1, OX, PPOX
RefSeq:   NC_000017.10 NG_011448.1 NT_010783.15
Ensembl:   ENSG00000161610
Entrez:   3060
Chromosome Mapping:   17q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.140000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download