PrimePCR™ SYBR® Green Assay: EIF4E3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029083

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EIF4E3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EIF4E3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013869
Assay Design:   Intron-spanning
Chromosome Location:   3:71739279-71748790question
Amplicon Length:   161
Splice Variants Targeted:   ENST00000295612 ENST00000421769 ENST00000497838 ENST00000448225 ENST00000389826 ENST00000425534

Gene Information

EIF4E3 belongs to the EIF4E family of translational initiation factors that interact with the 5-prime cap structure of mRNA and recruit mRNA to the ribosome (Joshi et al. 2004 [PubMed 15153109]).[supplied by OMIM Mar 2008]

Gene Symbol:   EIF4E3
Gene Name:   eukaryotic translation initiation factor 4E family member 3
Aliases:   MGC39820, MGC86971
RefSeq:   NC_000003.11 NT_022459.15
Ensembl:   ENSG00000163412
Entrez:   317649
Chromosome Mapping:   3p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.350000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download