PrimePCR™ SYBR® Green Assay: TBL1XR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013861
Assay Design:   Intron-spanning
Chromosome Location:   3:176771565-176782780question
Amplicon Length:   185
Splice Variants Targeted:   ENST00000430069 ENST00000457928 ENST00000352800 ENST00000437738 ENST00000450267 ENST00000431674 ENST00000422066 ENST00000443315 ENST00000422442

Gene Information

The protein encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction RNA processing gene regulation vesicular trafficking cytoskeletal assembly and may play a role in the control of cytotypic differentiation. [provided by RefSeq Jul 2008]

Gene Symbol:   TBL1XR1
Gene Name:   transducin (beta)-like 1 X-linked receptor 1
Aliases:   C21, DC42, FLJ12894, IRA1, TBLR1
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000177565
Entrez:   79718
UniGene:   Hs.581171
Chromosome Mapping:   3q26.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 35.130000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download