This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029077
PrimePCR™ PreAmp for SYBR® Green Assay: PCGF1, Human
PrimePCR™ Template for SYBR® Green Assay: PCGF1, Human
PCGF1 is a mammalian homolog of the Drosophila polycomb group genes which act as transcriptional repressors to regulate anterior-posterior patterning in early embryonic development (Nunes et al. 2001 [PubMed 11287196]). See also PCGF2 (MIM 600346).[supplied by OMIM Aug 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.