PrimePCR™ SYBR® Green Assay: PCGF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029077

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013856
Assay Design:   Intron-spanning
Chromosome Location:   2:74733958-74734746question
Amplicon Length:   174
Splice Variants Targeted:   ENST00000233630

Gene Information

PCGF1 is a mammalian homolog of the Drosophila polycomb group genes which act as transcriptional repressors to regulate anterior-posterior patterning in early embryonic development (Nunes et al. 2001 [PubMed 11287196]). See also PCGF2 (MIM 600346).[supplied by OMIM Aug 2008]

Gene Symbol:   PCGF1
Gene Name:   polycomb group ring finger 1
Aliases:   2010002K04Rik, FLJ43754, MGC10882, NSPC1, RNF3A-2, RNF68
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000115289
Entrez:   84759
UniGene:   Hs.316750
Chromosome Mapping:   2p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.720000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download