This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029030
PrimePCR™ PreAmp for SYBR® Green Assay: GZMB, Human
PrimePCR™ Template for SYBR® Green Assay: GZMB, Human
Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize bind and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.