This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029002
PrimePCR™ PreAmp for SYBR® Green Assay: SURF6, Human
PrimePCR™ Template for SYBR® Green Assay: SURF6, Human
This gene is located in the surfeit gene cluster a group of very tightly linked genes that do not share sequence similarity. The gene demonstrates features of a housekeeping gene being ubiquitously expressed and the encoded protein has been localized to the nucleolus. The protein includes motifs found in both the mouse and fish orthologs which suggests a putative function as a nucleolar-matrix protein with nucleic acid-binding properties based on characteristics determined in mouse. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.