PrimePCR™ SYBR® Green Assay: C10orf2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013706
Assay Design:   Intron-spanning
Chromosome Location:   10:102750682-102752996question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000311916 ENST00000370228

Gene Information

This gene encodes a hexameric DNA helicase which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma is thought to play a key role in mtDNA replication. The protein localizes to the mitochondrial matrix and mitochondrial nucleoids. Mutations in this gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq Aug 2009]

Gene Symbol:   C10orf2
Gene Name:   chromosome 10 open reading frame 2
Aliases:   ATXN8, FLJ21832, IOSCA, MTDPS7, PEO, PEO1, PEOA3, SANDO, SCA8, TWINL
RefSeq:   NC_000010.10 NG_011646.1 NT_030059.13 NG_012624.1
Ensembl:   ENSG00000107815
Entrez:   56652
UniGene:   Hs.22678
Chromosome Mapping:   10q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.180000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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