This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028986
PrimePCR™ PreAmp for SYBR® Green Assay: CD44, Human
PrimePCR™ Template for SYBR® Green Assay: CD44, Human
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands such as osteopontin collagens and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation recirculation and homing hematopoiesis and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms however the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein and may be related to tumor metastasis. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.