PrimePCR™ SYBR® Green Assay: RFXANK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013661
Assay Design:   Intron-spanning
Chromosome Location:   19:19303797-19304817question
Amplicon Length:   153
Splice Variants Targeted:   ENST00000421262 ENST00000456252 ENST00000303088 ENST00000407360 ENST00000543652 ENST00000540981 ENST00000541873 ENST00000392324 ENST00000353145

Gene Information

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene along with regulatory factor X-associated protein and regulatory factor-5 forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter this complex associates with the non-DNA-binding factor MHC class II transactivator which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II complementation group B. Two transcript variants encoding different isoforms have been described for this gene with only one isoform showing activation activity. [provided by RefSeq Jul 2008]

Gene Symbol:   RFXANK
Gene Name:   regulatory factor X-associated ankyrin-containing protein
Aliases:   ANKRA1, BLS, F14150_1, MGC138628, RFX-B
RefSeq:   NC_000019.9 NG_007432.1 NT_011295.11
Ensembl:   ENSG00000064490
Entrez:   8625
UniGene:   Hs.296776
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.540000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download